孙建和 职      称：教授（Tenured Professor） 所属学科：预防兽医学  研究方向： （1）分子病毒学；（2）分子免疫学 电话/传真：021-34206926 电子信箱：sunjhe@sjtu.edu.cn 联系地址：必威A楼3-205室

 

个人简介： 

孙建和博士，男，江苏海安人。上海交通大学长聘教授（Tenured Professor）、博士生导师。上海市农业领军人才，上海市曙光学者。担任教育部高等学校动物医学类专业教学指导委员会委员、中国畜牧兽医学会理事、中国微生物学会兽医微生物学专业委员会委员、上海市畜牧兽医学会副理事长。担任《微生物学报》编委、《微生物与感染》常务编委、《中国兽医科学》编委等。 

 

研究兴趣：   

动物抗病毒天然免疫信号转导机制与应用策略 

天然免疫在机体抗病毒策略中发挥极其重要的作用。在病毒感染后，宿主细胞可通过模式识别受体（PRR）识别病原相关分子模式（PAMP），进而通过信号转导启动一系列免疫级联反应，调控天然免疫应答，维持免疫稳态，发挥抗病毒作用。鉴于家禽与哺乳动物的天然免疫存在显著差异，我们基于比较免疫学，发掘家禽新的天然免疫信号分子，揭示信号转导途径，阐明抗病毒的分子机制与应用策略。

 

噬菌体及其编码蛋白介导细菌裂解的分子机制与抗菌应用

在临床病原细菌对抗生素严重耐药和多重耐药的背景下，聚焦噬菌体裂菌相关功能蛋白——裂解酶（lysin）和穿孔素（holin），发掘噬菌体的抗菌应用潜力。通过对噬菌体裂解酶的晶体结构解析，阐明其识别和水解细菌肽聚糖的酶学基础；通过对噬菌体穿孔素的膜拓扑结构解析，揭示噬菌体触发细菌裂解的分子机制。通过挖掘、重构广谱、高效的噬菌体及其裂菌分子，创建高效、绿色抗菌制剂研究与应用技术平台。

 

近期代表性论文：

1. Jie Wang, Zhenyu Lin , Qiuju Liu , Feiyu Fu, Zhaofei Wang, Jingjiao Ma, Hengan Wang,Yaxian Yan, Yuqiang Cheng* and Jianhe Sun* . Bat Employs a Conserved MDA5 Gene to Trigger Antiviral Innate Immune Responses. Frontiers in Immunology, 2022.

2. Zhenyu Lin, Jie Wang, Shurui Zhao, Yanlin Li , Yanhe Zhang , Yue Wang , Yaxian Yan, Yuqiang Cheng *, Jianhe Sun**. Goose IRF7 is involved in antivirus innate immunity by mediating IFN activation. Developmental and Comparative Immunology, 2022.

3. Zhenyu Lin, Jie Wang, Nian Zhang, Jianshu Yi, Zhaofei Wang, Jingjiao Ma, Hengan Wang, Yaxian Yan, Kun Qian, Jianhe Sun* and Yuqiang Cheng*. Functional characterization of goose IRF1 in IFN induction and anti‑NDV infection. Veterinary Research, 2022.

4. Lixiang Xie, Guanlong Xu, Lingxiang Xin, Zhaofei Wang, Rujuan Wu, Mingqing Wu, Yuqiang Cheng, Hengan Wang, Yaxian Yan, Jingjiao Ma *, and Jianhe Sun * Eurasian Avian-like M1 Plays More Important Role than M2 in Pathogenicity of 2009 Pandemic H1N1 Influenza Virus in Mice. Viruses, 2021.

5. Hou S, Ma J, Cheng Y, Wang H, Sun J, Yan Y. The toxicity mechanisms of DON to humans and animals and potential biological treatment strategies. Critical Reviews in Food Science and Nutrition, 2021.

6. Zhenyu Lin, Jie Wang, Wenxian Zhu, Xiangyu Yu, Zhaofei Wang, Jingjiao Ma, Hengan Wang, Yaxian Yan, Jianhe Sun, Yuqiang Cheng. Chicken DDX1 Acts as an RNA Sensor to Mediate IFN-β Signaling Pathway Activation in Antiviral Innate Immunity. Frontiers in Immunology, 2021.

7. Wenxian Zhu, Yuqiang Cheng, Zhaofei Wang, Likai Ji, Jingjiao Ma, Yaxian Yan, Hengan Wang, Jianhe Sun. The identification of a shorter cyclic GMP-AMP synthase gene from chickens and bioinformatics analysis of its potential signaling in IFN regulation. Developmental and comparative immunology, 2021.

8. Jingjiao Ma, Mingqing Wu, Zhaofei Wang, Denghui Yang, Silu Hou, Yuqiang Cheng, Hengan Wang, Yaxian Yan, Jianhe Sun. Pre-exposure to Streptococcus suis improved survival of influenza virus co-infection in mice. Veterinary Microbiology, 2021.

9. Zhaofei Wang, Yibing Xue, Ya Gao, Yuanping Liu, Mengting Guo, Xinwei Zou, Yuqiang Cheng, Jingjiao Ma, Hengan Wang, Jianhe Sun and Yaxian Yan. Phage vB_PaeS-PAJD-1 rescues murine mastitis infected with multidrug-resistant Pseudomonas aeruginosa [J]. Frontiers in Cellular and Infection Microbiology, 2021.

10. Mengting Guo, Ya Gao, Yibing Xue, Yuanping Liu, Xiaoyan Zeng, Yuqiang Cheng, Jingjiao Ma, Hengan Wang, Jianhe Sun, Zhaofei Wang and Yaxian Yan. Bacteriophage cocktails protect dairy cows against mastitis caused by drug resistant Escherichia coli infection [J]. Frontiers in Cellular and Infection Microbiology, 2021.

11. Zhaofei Wang, Mengting Guo, Licheng Kong, Ya Gao, Jingjiao Ma, Yuqiang Cheng, Henan Wang, Yaxian Yan, Jianhe Sun*. TLR4 Agonist Combined with Trivalent Protein JointS of Streptococcus suis Provides Immunological Protection in Animals, Vaccines (Basel), 2021.

12. Jingjiao Ma, Rujuan Wu, Guanlong Xu, Yuqiang Cheng, Zhaofei Wang, Hengan Wang, Yaxian Yan, Jinxiang Li and Jianhe Sun*. Acetylation at K108 of the NS1 protein is important for the replication and virulence of influenza virus，Veterinary Research, 2020.

13. Likai Ji, Na Wang, Jingjiao Ma, Yuqiang Cheng, Hengan Wang, Jianhe Sun, Yaxian Yan. Porcine deltacoronavirus nucleocapsid protein species specifically suppressed IRF7 induced type I interferon production via ubiquitin proteasomal degradation pathway，Veterinary Microbiology, 2020.

14. Denghui Yang, ZhaofeiWang, Jingjiao Ma, Qiang Fu, LifeiWu, Hengan Wang, Shaohui Wang, Yaxian Yan, and Jianhe Sun. Glycine Cleavage System and cAMP Receptor Protein Co-Regulate CRISPR/cas3 Expression to Resist Bacteriophage, Viruses, 2020.

15. Silu Hou, Jingjiao Ma, Yuqiang Cheng, Hengan Wang, Jianhe Sun, Yaxian Yan. One-step rapid detection of fumonisin B-1, dexyonivalenol and zearalenone in grains，Food Control, 2020.

16. Silu Hou, Jingjiao Ma, Yuqiang Cheng, Hengan Wang, Jianhe Sun, Yaxian Yan. Quantum dot nanobead-based fluorescent immunochromatographic assay for simultaneous quantitative detection of fumonisin B-1, dexyonivalenol, and zearalenone in grains，Food Control, 2020.

17. Yuqiang Cheng, Wenxian Zhu, Chan Ding, Qiaona Niu, Hengan Wang, Yaxian Yan, and Jianhe Sun*. IRF7 Is Involved in Both STING and MAVS Mediating IFN-β Signaling in IRF3-Lacking Chickens. Journal of Immunology, 2019.

18. Jingjiao Ma, Rujuan Wu, Ye Tian, Min Zhang, Weili Wang, Yujie Li, Fulin Tian, Yuqiang Cheng, Yaxian Yan, Jianhe Sun: Isolation and characterization of an Aves polyomavirus 1 from diseased budgerigars in China. Veterinary Microbiology,  2019.

19. Zhaofei Wang, Jingjiao Ma, Jian Wang, Denghui Yang, Licheng Kong, Qiang Fu, Yuqiang Cheng, Hengan Wang, Yaxian Yan and Jianhe Sun: Application of the Phage Lysin Ply5218 in the Treatment of Streptococcus suis Infection in Piglets. Viruses, 2019.

20. Qiaona Niu, Yuqiang Cheng, Hengan Wang, Yaxian Yan and Jianhe Sun*: Chicken DDX3X Activates IFN-β via the chSTING-chIRF7-IFN-β Signaling Axis. Frontiers in Immunology, 2019.

21. Yuqiang Cheng, Minxiang Lun, Yunxia Liu, Hengan Wang, Yaxian Yan and Jianhe Sun*: CRISPR/Cas9-Mediated Chicken TBK1 Gene Knockout and Its Essential Role in STING-Mediated IFN-β Induction in Chicken Cells. Frontiers in Immunology, 2019.

22. Yuqiang Cheng, Yunxia Liu, Shuduan Shi, Qiaona Niu, Wenxian Zhu, Zhaofei Wang, Jingjiao Ma, Hengan Wang, Yaxian Yan* and Jianhe Sun*: Functional Characterization of Duck STING in IFN-b Induction and Anti-H9N2 Avian Influenza Viruses Infections. Frontiers in Immunology, 2019.

23. Ji Likai, Li Shasha, Zhu Wenxian, Ma Jingjiao, Sun Jianhe, Wang Hengan and Yan Yaxian*: Porcine Deltacoronavirus Nucleocapsid Protein Suppressed IFN-β Production by Interfering Porcine RIG-I dsRNA-Binding and K63-Linked Polyubiquitination. Frontiers in Immunology, 2019.

24. Jingjiao Ma, Ye Tian, Min Zhang, Yujie Li, Weili Wang, Fulin Tian, Yuqiang Chen, Yaxian Yan, Jianhe Sun*: Establishment of rapid detection method and surveillance of budgerigar fledgling disease virus using a TaqMan Real-Time PCR. Molecular and Cellular Probes, 2019.

25.  Wang Z, Kong L, Liu Y, Fu Q, Cui Z, Wang J, Ma J, Wang H, Yan Y, Sun J*: A Cell-Penetrating Peptide Fused Phage Lysin Kills Intracellular MRSA in Keratinocytes and Treatment for Skin Infections of Mice. Applied and environmental microbiology, 2018.

26. Yunxia Liu, Yuqiang Cheng, Wenya Shan, Jingjiao Ma, Hengan Wang, Jianhe Sun, Yaxian Yan*: Chicken interferon regulatory factor 1 (IRF1) involved in antiviral innate immunity via regulating IFN-β production. Developmental and comparative immunology, 2018.

27. Cheng Y, Sun Y, Wang H, Yan Y, Ding C, Sun J*: Chicken STING Mediates Activation of the IFN Gene Independently of the RIG-I Gene. Journal of immunology, 2015.

28. Sun Jianhe, Cai Y, Moll WD, Guo P: Controlling bacteriophage phi29 DNA-packaging motor by addition or discharge of a peptide at N-terminus of connector protein that interacts with pRNA. Nucleic acids research, 2006.

 

代表性授权发明专利：

1. 孙建和，孔里程，严亚贤，王兆飞，杨登辉。一种猪链球菌B细胞优势表位串联疫苗及其制备方法，中国，ZL201811353476.8, 2021-08-10

2. 孙建和，刘云霞，程玉强，严亚贤。利用双荧光素酶报告基因检测鸭IFN-β启动子活性的方法， 中国，ZL 201711195597.X, 2021-06-18

3. 孙建和，程玉强，伦敏翔，严亚贤。靶向敲除鸡IRF7基因的方法及其在疫苗制备中的应用，中国，ZL 201910352557.4，2021-06-18

4. 孙建和，王兆飞，严亚贤，傅强。复合型胞内金黄色葡萄球菌抗菌制剂及其制备和应用，中国，ZL201910059452X，2021-04-02

5. 孙建和，程玉强，朱雯娴，严亚贤。鸡TBK1基因的小干扰RNA质粒的构建方法及用途，中国，ZL 201810136202.7，2021-03-09

 

代表性专著或译著：

1. 胡福泉、童贻刚 主编,《噬菌体学：从理论到实践》，2021，上海科学出版社 (ISBN 978-7-030-69286-3)，（孙建和参编，负责撰写第5章：噬菌体复制的基因调控）

2. 陆承平、吴宗福 主编，《猪链球菌病》，2015，中国农业出版社（ISBN978-7-109-20686-1），（孙建和参编，负责撰写第5章和第6章）

3. Avraham Rasooly, Ben Prick ril, Methods in molecular biology 1571: Biosensors and Biodetection, Methods and Protocols. Humana Press. 2017  (孙建和 参编第26章)

4. 陈继明、马洪超、陆承平 主译，孙建和 副主译，《兽医微生物及所致传染病》（第2版），中国农业出版社，2015.（孙建和负责翻译第7、8、9、10、11、12、13章）